JWH 249 is primarily utilized in scientific research to study the endocannabinoid system and its interactions with cannabinoid receptors. Researchers employ it to investigate the pharmacological effects of synthetic cannabinoids, particularly their binding affinity and activity at CB1 and CB2 receptors. This helps in understanding the potential therapeutic applications and risks associated with cannabinoid compounds. Additionally, JWH 249 is used in forensic toxicology to identify and analyze synthetic cannabinoids in biological samples, aiding in the detection of substance abuse. Its role in these studies contributes to the development of safer and more effective cannabinoid-based medications.