JWH 250 N-(5-hydroxypentyl) metabolite is primarily studied in the context of forensic and clinical toxicology. Its application is significant in identifying and analyzing synthetic cannabinoid use, as it serves as a biomarker in biological samples such as urine or blood. This metabolite helps in confirming the consumption of JWH 250, a synthetic cannabinoid, which is often abused for its psychoactive effects. Detection of this metabolite is crucial for law enforcement and healthcare professionals to assess drug exposure, investigate cases of drug abuse, and provide appropriate medical or legal interventions. Additionally, it aids in research to understand the metabolism and pharmacokinetics of synthetic cannabinoids, contributing to the development of better detection methods and harm reduction strategies.