JWH 251 is primarily used in scientific research to study the endocannabinoid system and its interactions with synthetic cannabinoids. Researchers utilize it to understand the binding affinity and activation of cannabinoid receptors, particularly CB1 and CB2, which play a role in various physiological processes. It is also employed in pharmacological studies to explore its potential effects on pain, inflammation, and neurological disorders. Due to its structural similarity to other synthetic cannabinoids, JWH 251 serves as a reference compound in forensic and toxicological analyses to identify and characterize related substances in legal and regulatory investigations.