NVP-TNKS656 is primarily utilized in the field of cancer research, particularly in the study of Wnt signaling pathways. It functions as a potent and selective inhibitor of tankyrase enzymes, which play a critical role in regulating these pathways. By inhibiting tankyrase, NVP-TNKS656 helps researchers investigate the mechanisms underlying tumor growth and progression, especially in cancers where Wnt signaling is dysregulated. This compound is often employed in preclinical studies to assess its potential as a therapeutic agent, aiming to develop targeted treatments for colorectal cancer and other malignancies driven by aberrant Wnt signaling. Its application extends to cellular assays and animal models to evaluate efficacy, safety, and pharmacokinetics, providing valuable insights for drug development.