Tetrazine-Ph-acid is widely used in bioorthogonal chemistry due to its fast and selective reactivity with strained alkenes or alkynes. It is particularly valuable in labeling and imaging applications, where it enables the precise tagging of biomolecules in live cells or tissues without interfering with natural biological processes. Its high reaction efficiency makes it suitable for time-sensitive studies, such as tracking dynamic cellular events. Additionally, it is employed in drug delivery systems, where it facilitates the targeted release of therapeutic agents. The compound is also utilized in material science for surface functionalization and the development of smart materials.