This compound is widely utilized in bioorthogonal chemistry, particularly in click chemistry reactions. Its tetrazine moiety enables rapid and selective reactions with trans-cyclooctene (TCO) or other strained alkenes, making it valuable for labeling biomolecules in live cells or tissues without interfering with biological processes. It is commonly employed in the development of targeted drug delivery systems, where it facilitates the conjugation of therapeutic agents to specific biomolecules. Additionally, it is used in the imaging and tracking of cellular processes, as the tetrazine group can be labeled with fluorescent probes for real-time visualization. Its applications also extend to the field of proteomics, where it aids in the identification and study of protein interactions and functions.