This chemical is primarily used in advanced bioimaging and bioconjugation applications. Its structure, featuring a tetrazine group, makes it highly reactive in inverse electron-demand Diels-Alder (IEDDA) reactions, enabling rapid and specific labeling of biomolecules. This property is particularly valuable in live-cell imaging, where it allows for the tracking of cellular processes in real time with minimal background interference.

Additionally, the xanthylium moiety provides strong fluorescence, making it suitable for super-resolution microscopy techniques. This enhances the ability to study subcellular structures and dynamics with high precision. The compound is also utilized in the development of targeted drug delivery systems, where its reactive tetrazine group can be used to attach therapeutic agents to specific cells or tissues.

In research, it serves as a versatile tool for studying protein-protein interactions, enzyme activity, and other molecular events. Its stability and reactivity under physiological conditions make it a reliable choice for in vitro and in vivo applications. Overall, this chemical plays a crucial role in advancing biomedical research and therapeutic strategies.