This chemical is primarily used in bioorthogonal chemistry, where it serves as a key component in click chemistry reactions. Its tetrazine group enables rapid and selective conjugation with trans-cyclooctene (TCO) or other dienophiles, making it highly valuable for labeling and imaging applications in biological systems. It is often employed in the development of targeted drug delivery systems, as it allows for precise attachment of therapeutic agents to specific biomolecules. Additionally, it is utilized in the study of protein-protein interactions and cellular processes, where its efficient reactivity facilitates real-time tracking and visualization. Its water-soluble polyethylene glycol (PEG) linker enhances biocompatibility, making it suitable for in vivo applications.