This chemical is primarily used in bioorthogonal chemistry, particularly in click chemistry reactions. Its tetrazine group enables rapid and selective reactions with strained alkenes or alkynes, making it valuable for labeling biomolecules in live cells without interfering with native biochemical processes. It is often employed in the development of targeted drug delivery systems, where it facilitates the attachment of therapeutic agents to specific cellular targets. Additionally, it is utilized in imaging techniques, such as fluorescence microscopy, to visualize and track biomolecules in real-time. Its unique structure also supports applications in material science, where it can be used to modify surfaces or create functionalized polymers.