This chemical is primarily utilized in the field of bioconjugation and click chemistry, particularly in the synthesis of biomolecules. Its tetrazine group enables efficient and rapid ligation with trans-cyclooctene (TCO) derivatives, making it valuable for labeling proteins, antibodies, and other biological targets. This property is especially useful in live-cell imaging, where it allows for real-time tracking of biomolecules with minimal interference to cellular processes. Additionally, it is employed in the development of targeted drug delivery systems, where its reactivity ensures precise attachment of therapeutic agents to specific cellular targets. The compound’s polyethylene glycol (PEG) linker enhances solubility and biocompatibility, making it suitable for applications in aqueous environments. Overall, it plays a critical role in advancing research in chemical biology, diagnostics, and therapeutic development.