This compound is primarily utilized in bioorthogonal chemistry, where it serves as a key reagent for click chemistry reactions. Its tetrazine group enables rapid and selective reactions with strained alkenes or alkynes, making it valuable for labeling biomolecules in live cells or tissues. This property is particularly useful in imaging and tracking biological processes in real-time without interfering with native cellular functions. Additionally, it is employed in the development of targeted drug delivery systems, where it facilitates the conjugation of therapeutic agents to specific biomolecules. Its applications also extend to materials science, where it is used to modify surfaces or create functionalized polymers for advanced materials.