This chemical is primarily utilized in bioorthogonal chemistry, where it serves as a key component in click chemistry reactions. Its tetrazine group enables rapid and selective conjugation with trans-cyclooctene (TCO) or other dienophiles, making it valuable for labeling biomolecules such as proteins, nucleic acids, and lipids in live cells or complex biological systems. This property is particularly useful in imaging and tracking studies, where it allows for real-time visualization of dynamic processes. Additionally, it is employed in drug delivery systems to create targeted conjugates, enhancing the specificity and efficiency of therapeutic agents. Its unique reactivity also supports the development of advanced materials and surface modifications in nanotechnology.