Methyl thiobutyrate is widely used in the flavor and fragrance industry due to its strong, fruity, and slightly sulfurous aroma. It is commonly employed as a flavoring agent in food products, particularly in creating tropical fruit flavors such as pineapple, strawberry, and passionfruit. Additionally, it serves as a key ingredient in the formulation of perfumes and scented products, where it contributes to complex, exotic, and sweet olfactory profiles. Its versatility also extends to the synthesis of other organic compounds in chemical research and industrial applications.