Used as a protecting group reagent in peptide synthesis, particularly for the selective protection of thiol groups during the assembly of complex disulfide-rich proteins. Its primary application lies in facilitating the controlled formation of disulfide bonds by temporarily masking reactive sulfhydryl groups, which can later be deprotected under mild conditions. The tert-butyl carbamate (Boc) group provides stability during synthetic steps while allowing orthogonal deprotection strategies. Commonly employed in pharmaceutical research and development of bioactive peptides where precise disulfide pairing is critical for biological activity. Also useful in bioconjugation chemistry and the synthesis of cysteine-containing biomolecules.