S-Acetylthioglycolic acid N-hydroxysuccinimide ester is widely used in bioconjugation chemistry for modifying biomolecules. It serves as a key reagent in the preparation of thiol-reactive crosslinkers, enabling the attachment of peptides, proteins, or antibodies to various surfaces or other molecules. Its acetyl group provides stability, preventing premature reactions, while the N-hydroxysuccinimide ester facilitates efficient coupling with primary amines. This compound is particularly valuable in the development of diagnostic assays, targeted drug delivery systems, and functionalized nanoparticles. Its ability to introduce thiol groups into biomolecules makes it essential for creating stable and site-specific conjugates in biochemical research and therapeutic applications.