Acetone thiosemicarbazone is primarily utilized in the field of medicinal chemistry due to its potential biological activities. It is studied for its antimicrobial properties, showing effectiveness against various bacterial and fungal strains. Additionally, it is explored for its role in cancer research, as it exhibits antitumor activity by inhibiting specific enzymes involved in cell proliferation. The compound is also investigated for its chelating properties, which can be useful in metal ion detoxification and in the development of metal-based drugs. Furthermore, it serves as a precursor in the synthesis of more complex thiosemicarbazone derivatives, which are evaluated for their therapeutic potential in treating infectious diseases and certain types of cancer.