Thiosemicarbazide is widely used in analytical chemistry as a reagent for the detection and determination of various metal ions, such as copper, nickel, and cobalt, due to its ability to form stable complexes. It serves as a key intermediate in the synthesis of heterocyclic compounds, including thiadiazoles and triazoles, which are important in pharmaceutical and agrochemical industries. In the field of materials science, it is utilized in the preparation of coordination polymers and metal-organic frameworks (MOFs) with potential applications in catalysis, gas storage, and separation processes. Additionally, thiosemicarbazide derivatives are explored for their biological activities, including antimicrobial, antiviral, and anticancer properties, making them valuable in medicinal chemistry research.