Used in biomedical research to measure thyroxine (T4) hormone levels in mouse serum or plasma samples. Helps in studying thyroid function, endocrine disorders, and the impact of drugs or environmental factors on the hypothalamic-pituitary-thyroid axis in mouse models. Commonly applied in preclinical studies, toxicology research, and metabolic disease investigations. Provides a sensitive and specific quantitative analysis, enabling researchers to assess thyroid health and hormonal balance in experimental animals.