SPD304 dihydrochloride is primarily utilized in biochemical research as a potent inhibitor of tumor necrosis factor alpha (TNF-α). It is particularly valuable in studies focused on understanding the role of TNF-α in inflammatory diseases, autoimmune disorders, and cancer. By inhibiting TNF-α trimerization, it helps researchers explore the signaling pathways and cellular responses associated with this cytokine. Additionally, SPD304 dihydrochloride is used in drug discovery and development processes to identify and test potential therapeutic agents targeting TNF-α-related conditions. Its application extends to in vitro and in vivo experiments, providing insights into the mechanisms of inflammation and immune regulation.