This chemical is primarily used in bioconjugation and click chemistry applications due to its functional groups, which enable efficient and selective binding to biomolecules. The maleimide (Mal) group reacts specifically with thiol groups (-SH) present in cysteine residues of proteins, allowing for stable covalent attachment. The two TCO (trans-cyclooctene) groups are highly reactive toward tetrazine, facilitating rapid and bioorthogonal click reactions. This dual functionality makes it a versatile tool for labeling, imaging, and targeting biomolecules in research and therapeutic development. Its PEG (polyethylene glycol) spacers enhance solubility and reduce steric hindrance, improving the efficiency of conjugation reactions in aqueous environments. This compound is particularly useful in the development of antibody-drug conjugates (ADCs), targeted drug delivery systems, and molecular imaging probes.