Pifithrin-β is widely used in research to study its role as an inhibitor of p53, a protein critical in regulating cell cycle and apoptosis. Its application is particularly significant in cancer research, where it helps scientists understand the mechanisms of tumor suppression and the potential for therapeutic intervention. By temporarily blocking p53 activity, researchers can investigate how cancer cells evade apoptosis and develop resistance to treatments. Additionally, it is utilized in studies exploring tissue protection during chemotherapy or radiation, as inhibiting p53 can reduce damage to healthy cells. Pifithrin-β is also employed in neurobiology to examine its neuroprotective effects in models of neurodegenerative diseases and brain injury. Its ability to modulate cellular stress responses makes it a valuable tool in experimental biology and drug development.