Used as a cationic lipid in gene delivery systems, enabling efficient transfection of DNA and RNA into cells. Its positive charge interacts with negatively charged nucleic acids, forming lipoplexes that fuse with cell membranes to enhance cellular uptake. Commonly applied in research for in vitro and in vivo gene expression studies, vaccine development, and gene therapy. Also utilized in liposomal formulations to improve stability and targeting of therapeutic nucleic acids. Shows effectiveness in both dividing and non-dividing cells, making it valuable in diverse biomedical applications.