Hexadimethrine bromide is widely used as a cationic polymer in molecular biology, particularly for enhancing the efficiency of DNA transfection in cell cultures. It acts as a polycationic agent, binding to the negatively charged DNA molecules, which helps in forming complexes that can more easily penetrate cell membranes. This property makes it valuable in genetic engineering and research applications where introducing foreign DNA into cells is necessary. In addition to its role in transfection, it is also employed in the purification of nucleic acids. It aids in the precipitation of DNA and RNA from solutions, facilitating their isolation and subsequent analysis. This is particularly useful in laboratory settings where high-purity nucleic acids are required for experiments. Furthermore, hexadimethrine bromide finds application in the field of biotechnology for modifying surfaces to improve cell adhesion. By coating surfaces with this polymer, researchers can create a more favorable environment for cell growth and attachment, which is crucial for various cell culture studies and tissue engineering applications.