Western Blot Transfer Buffer (10×) is primarily used in the transfer step of Western blotting, a technique for detecting specific proteins in a sample. The buffer facilitates the efficient movement of proteins from a gel to a membrane, typically made of nitrocellulose or PVDF, after electrophoresis. This transfer is crucial for subsequent antibody-based detection of target proteins. The 10× concentration is diluted to 1× with water and methanol before use, ensuring optimal conductivity and protein binding to the membrane. It is widely employed in molecular biology, biochemistry, and medical research for studying protein expression, post-translational modifications, and protein interactions.