ML213 is primarily utilized in research settings as a potent and selective activator of the TRPC5 ion channel. This makes it a valuable tool for studying the physiological and pathological roles of TRPC5 in various biological systems. Researchers employ ML213 to investigate its effects on neuronal signaling, cardiovascular function, and kidney physiology, as TRPC5 channels are implicated in these processes. Additionally, it is used to explore potential therapeutic applications, particularly in conditions like anxiety, depression, and kidney diseases, where TRPC5 activity may play a critical role. Its specificity allows for precise modulation of TRPC5 channels, aiding in the development of targeted treatments and a deeper understanding of channel-related mechanisms.