TRPM4-IN-1 is primarily used in research settings to study the role of TRPM4 channels in various physiological and pathological processes. It is applied as a selective inhibitor of TRPM4, a calcium-activated non-selective cation channel, to investigate its involvement in cellular functions such as membrane potential regulation, calcium signaling, and cell volume control. Researchers utilize TRPM4-IN-1 to explore its potential therapeutic applications in conditions like stroke, cardiovascular diseases, and immune responses, where TRPM4 channels are implicated. Additionally, it aids in understanding the channel's role in cancer progression, as TRPM4 has been linked to tumor cell migration and metastasis. Its application in experimental models helps elucidate the mechanisms of TRPM4 activity and its impact on disease pathways.