Used in research settings as a peptide-based probe for studying immune responses, particularly in antigen presentation pathways. It serves as a model peptide to investigate MHC class I binding and T-cell receptor recognition due to its high affinity and stability. Commonly employed in immunological assays, including tetramer formation for flow cytometry to track antigen-specific T cells. Also utilized in vaccine development studies to evaluate cytotoxic T lymphocyte activity. The TFA salt form enhances solubility and handling in aqueous solutions.