Used in solid-phase peptide synthesis, particularly for constructing complex peptides containing disulfide bonds. The Acm (acetamidomethyl) protecting group stabilizes the thiol side chain during synthesis and can be selectively removed under mild conditions, allowing controlled disulfide bond formation. The Fmoc group enables orthogonal protection strategy, compatible with base-labile deprotection steps, making it ideal for automated peptide synthesis. Commonly applied in the production of therapeutic peptides, cyclic peptides, and peptide-based research tools where precise disulfide pairing is critical for biological activity.