Flumequine sodium is primarily used in veterinary medicine as an antimicrobial agent to treat bacterial infections in animals, especially in poultry and swine. It is effective against a range of Gram-negative and some Gram-positive bacteria. The compound is commonly administered through drinking water or feed to control infections such as colibacillosis, salmonellosis, and other enteric diseases. Due to concerns over antibiotic resistance, its use has been restricted or banned in several countries, particularly in food-producing animals, to prevent residues in meat and ensure food safety. It is also occasionally used in laboratory settings for selective isolation of microorganisms in culture media.