Used primarily as a photoactivatable crosslinking agent in biochemical and proteomic research. Upon exposure to UV light, the diazirine group generates highly reactive carbenes that insert into nearby C–H, O–H, or N–H bonds, enabling covalent linkage between interacting biomolecules. This property makes it valuable for studying protein–protein interactions, mapping binding sites, and identifying transient or weak molecular interactions in complex biological systems. The tert-butyl carbamate (Boc) protected amine allows for further synthetic modifications, making it useful in the design of photoaffinity probes and labeled ligands. Commonly incorporated into peptides, nucleotides, or small molecules to trace interactions in live cells or cell lysates with high spatial and temporal control.