Used primarily as a photoactivatable crosslinking agent in biochemical and proteomic research. Upon exposure to UV light, it generates highly reactive carbenes that form covalent bonds with nearby biomolecules, enabling the study of protein-protein interactions, ligand-receptor binding, and molecular proximity in complex biological systems. Its benzoyl chloride functional group allows for easy conjugation to amines or alcohols in target molecules, making it valuable for labeling and affinity tagging applications. The trifluoromethyl diazirine group provides enhanced stability and efficient photoactivation, ideal for time-resolved crosslinking experiments in live cells or in vitro assays.