Icterogenin is primarily used in biochemical research for its potential hepatoprotective and anti-inflammatory properties. It has been investigated for its role in modulating liver enzyme activity, particularly in models of liver injury and jaundice. Due to its structural similarity to certain triterpenoids, it shows promise in supporting liver detoxification pathways and reducing oxidative stress in hepatic tissues. It is also utilized as a reference compound in metabolic studies involving bile acid synthesis and steroid metabolism. Its application extends to the development of analytical methods for detecting liver function markers in preclinical studies.