Used in bioorthogonal chemistry for labeling and tracking biomolecules in live cells. Its alkyne group undergoes click reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), enabling selective conjugation with azide-containing probes. Commonly applied in metabolic labeling of proteins and glycans when introduced as a cellular metabolite. Also utilized in polymer chemistry to introduce functional handles into biobased materials. Supports research in chemical biology and drug discovery by facilitating the visualization and isolation of modified biomolecules.