JW55 is primarily used in research settings as a potent and selective inhibitor of the Wnt/β-catenin signaling pathway. This pathway plays a critical role in cell proliferation, differentiation, and stem cell regulation, and its dysregulation is associated with various cancers and fibrotic diseases. JW55 helps in studying the mechanistic aspects of this pathway by effectively suppressing β-catenin activity, making it a valuable tool in oncology and developmental biology research. It has shown potential in preclinical studies for inhibiting tumor growth, particularly in cancers where Wnt signaling is hyperactive, such as colorectal, liver, and breast cancers. Researchers also use JW55 to investigate tissue regeneration and fibrosis, as modulating Wnt signaling can influence scar formation and organ repair processes. Due to its specificity and cell permeability, JW55 is favored in cellular and animal models to validate therapeutic targets before drug development. However, it remains a research compound and is not used clinically.