Used in research to study cell signaling pathways, particularly the Notch signaling pathway, which plays a critical role in cell differentiation, development, and tissue homeostasis. This peptide fragment acts as a ligand for Notch receptors and is applied in studies related to stem cell biology, tissue regeneration, and cancer development. It helps in understanding how cell-to-cell communication influences cellular fate and can be used to modulate Notch activation in experimental models. Often employed in in vitro assays to trigger Notch signaling in co-culture systems or to investigate the effects of localized pathway activation.