Used as a fluorogenic substrate in enzymatic assays to detect protease activity, particularly for enzymes that cleave after leucine residues, such as leucine aminopeptidases. Upon enzymatic hydrolysis, it releases a highly fluorescent 7-amino-4-methylcoumarin product, allowing sensitive measurement of enzyme kinetics and inhibition in biochemical and cellular studies. Commonly applied in high-throughput screening, diagnostic assays, and research involving proteolytic enzyme function.