Lyticase from Bacillus subtilis is widely used in molecular biology and biotechnology for the enzymatic lysis of yeast and fungal cell walls. It enables efficient release of intracellular components such as DNA, RNA, proteins, and organelles without the need for harsh mechanical disruption methods. This makes it especially valuable in plasmid preparation, yeast transformation protocols, and the isolation of intact protoplasts for fusion experiments. Due to its specificity in degrading β-1,3-glucan linkages in cell walls, it provides a gentle and effective means for cell disruption, improving yield and purity in downstream applications. It is also employed in high-throughput screening and diagnostic procedures where rapid and consistent cell lysis is required.