Used to measure nitric oxide synthase (NOS) enzyme activity in mouse biological samples such as tissue homogenates, cell lysates, and serum. Commonly applied in research settings to study inflammatory responses, immune function, and vascular health, where nitric oxide plays a key signaling role. Enables quantitative analysis through enzyme-linked immunosorbent assay (ELISA), offering high sensitivity and specificity. Widely used in preclinical studies involving neurodegenerative diseases, cardiovascular disorders, and cancer to assess changes in NOS activity under experimental conditions.