Used primarily as a reference standard in analytical laboratories for the detection and quantification of organophosphorus compounds, especially in environmental and biological samples. It serves as a calibration standard in gas chromatography (GC) and liquid chromatography-mass spectrometry (LC-MS) to ensure accurate identification of pesticide metabolites and chemical warfare agent degradation products. Due to its reactivity, it is also employed in research settings to study phosphorylation reactions and nerve agent antidote development. Strict handling protocols are followed due to its high toxicity and potential use in regulated compound analysis.