Used in molecular biology and genomics research to isolate intact nuclei from cells or tissues. This buffer helps break open the cell membrane while preserving the nuclear membrane, allowing for the extraction of pure nuclei. It is commonly applied in assays such as ATAC-seq, ChIP-seq, and nuclear RNA analysis. The formulation supports the removal of cytoplasmic components and prevents degradation of nuclear contents, ensuring high-quality downstream analysis. Suitable for use with mammalian cells, plant tissues, and frozen samples.