NLN Medium is widely used in plant tissue culture, particularly for the micropropagation of orchids and other sensitive plant species. It provides a balanced composition of macro and micronutrients that support optimal growth during various stages of culture, including initiation, multiplication, and rooting. The formulation is especially effective for protocorm-like body (PLB) induction and somatic embryogenesis. Due to its low salt concentration and optimized nutrient ratios, NLN Medium helps reduce hyperhydricity (vitrification) in cultured tissues, improving the overall health and vigor of regenerated plants. It is commonly used in conservation programs and commercial orchid production where high efficiency and genetic stability are required.