N2-Benzoylguanosine is a protected nucleoside derivative used primarily as a building block in the chemical synthesis of oligonucleotides, especially for RNA. The N2-benzoyl protecting group shields the exocyclic amino group during solid-phase phosphoramidite synthesis, enabling efficient incorporation of guanosine residues while preventing unwanted side reactions. After synthesis, the protecting group is removed to yield standard guanosine in the final oligonucleotide. This compound is essential for producing high-purity synthetic nucleic acids applied in molecular biology research, gene therapy, diagnostics, and drug development.