Used primarily as a photoactivatable crosslinking agent in biochemical research. Its main application lies in photoaffinity labeling, where it helps identify molecular interactions between proteins, ligands, and nucleic acids. Upon UV irradiation, the diazirine group generates highly reactive carbenes that form covalent bonds with nearby molecules, capturing transient or weak interactions. The alkyne group enables downstream detection or purification via click chemistry, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC). This dual functionality makes it valuable for studying protein-protein interactions, mapping binding sites, and probing cellular pathways in complex biological systems.