1,2-Dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt), commonly known as DOGS-NTA-Ni, is a specialized phospholipid used for the immobilization of His-tagged proteins on lipid bilayers and membranes. The NTA headgroup chelates nickel ions, enabling specific and high-affinity binding to polyhistidine tags on recombinant proteins. This facilitates oriented protein attachment in supported lipid bilayers (SLBs), liposomes, or planar membranes, which is crucial for studying membrane protein structure, function, and interactions. It is widely applied in biophysical techniques such as surface plasmon resonance (SPR), quartz crystal microbalance (QCM-D), atomic force microscopy (AFM), and fluorescence microscopy. Additionally, it supports applications in biomimetic sensor development, cell-free protein reconstitution, and affinity-based purification systems in molecular biology and biochemistry research.