Used as a crosslinking agent in biochemical research for conjugating peptides or proteins due to its ability to react with thiol and amine groups. Its bifunctional bromoacetamide groups enable stable covalent bonds with cysteine residues, making it suitable for preparing protein-protein or protein-DNA conjugates. Also employed in the synthesis of cyclic peptides and bioconjugates where controlled spacing and stability are required. Additionally, it serves as an intermediate in the preparation of molecular probes and affinity labels.