Otophylloside B 4'''-O-α-L-cymaropyranoside is used in traditional medicine formulations for its anti-inflammatory and analgesic properties, particularly in otic preparations to treat ear-related infections and reduce swelling and discomfort in ear canal inflammations. It occurs naturally in plants traditionally used for ear drops and exhibits moderate antimicrobial activity against common pathogens. Ongoing research explores its role in modulating oxidative stress and supporting tissue protection in auditory systems. Additionally, molecular biology studies highlight its potential in inhibiting pro-inflammatory cytokines such as TNF-α and IL-6, as well as suppressing proliferation of certain cancer cells, positioning it as a promising lead compound for developing treatments for chronic inflammatory diseases and cancer.