Phenol citrate solution
Reagent
Code: #223502
Properties
The phenolic salt-citrate solution of 1EA was mixed with 3.0 mL of sodium nitroferrocyanide solution (10 g/L), 5.0 mL of phenol-ethanol solution, 6.5 mL of sodium hydroxide solution (240 g/L) and 50 mL of sodium tricate solution (400 g/L).
blur_circular Chemical Specifications
inventory_2
Storage & Handling
Storage
2-8°C
description Product Description
Phenol citrate solution is used as a solvent or medium in the extraction of nucleic acids, particularly in the isolation of DNA or RNA from cells or tissues. It possesses properties that disrupt cell membranes and nuclear envelopes, as well as denature proteins, allowing efficient separation from nucleic acids. After mixing and centrifugation, phase separation occurs, with nucleic acids in the aqueous phase, while proteins and other impurities remain in the phenol phase or at the interface. It is commonly used in molecular biology laboratories to prepare DNA or RNA samples for further analysis, such as PCR, sequencing, or gene expression studies.
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