Used in research to modulate the Wnt signaling pathway by specifically inhibiting PORCN, an enzyme essential for Wnt protein palmitoylation. This inhibition prevents Wnt ligand secretion, making it a valuable tool for studying Wnt-dependent processes such as cell proliferation, differentiation, and tissue development. Commonly applied in cancer research, particularly in models of tumors driven by aberrant Wnt signaling, including certain colorectal and pancreatic cancers. Also utilized in stem cell and developmental biology studies to dissect the role of Wnt signaling in tissue homeostasis and embryogenesis. Its selectivity allows for targeted pathway analysis in cellular and animal models.