Used in bioconjugation processes to link proteins, antibodies, or peptides with other molecules such as drugs, labels, or nanoparticles. Its primary application is in the development of antibody-drug conjugates (ADCs), where it enables controlled attachment through disulfide bond formation. The N-hydroxysuccinimide ester group reacts efficiently with primary amines on biomolecules, while the pyridyldithio group allows for thiol-disulfide exchange, facilitating reversible linkage. This makes it valuable in targeted drug delivery systems and diagnostic probes. Also employed in immobilizing biomolecules onto surfaces for biosensors or assay platforms due to its stable yet cleavable linker properties.