Pulsatilloside C is primarily studied for its potential immunomodulatory and anti-inflammatory properties. It has shown activity in regulating immune responses by influencing cytokine production, particularly suppressing pro-inflammatory mediators such as TNF-α and IL-6. This makes it a candidate of interest in research related to autoimmune diseases and chronic inflammatory conditions.

It is also being explored for its antiviral effects, especially in inhibiting the replication of certain enveloped viruses, likely due to its ability to interact with cell membranes or viral envelopes. Its saponin structure contributes to membrane permeability effects, which may enhance adjuvant activity in vaccine development.

Additionally, Pulsatilloside C has been investigated for use in traditional medicine formulations, where it contributes to the overall therapeutic effect of plant extracts, particularly in treatments aimed at respiratory and allergic conditions. However, most applications remain in the preclinical research stage, with ongoing studies to determine efficacy, safety, and mechanisms of action.